Antisense Reduction of NADP-Malic Enzyme in Flaveriabidentis Reduces Flow of CO 2 through the C 4 Cycle
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Pengelly, Jasper; Tan, Jackie; Furbank, Robert Thomas; von Caemmerer, Susanne
Description
An antisense construct targeting the C4 isoform of NADP-malic enzyme (ME), the primary enzyme decarboxylating malate in bundle sheath cells to supply CO2 to Rubisco, was used to transform the dicot Flaveria bidentis. Transgenic plants (a-NADP-ME) exhibited a 34% to 75% reduction in NADP-ME activity relative to the wild type with no visible growth phenotype. We characterized the effect of reducing NADP-ME on photosynthesis by measuring in vitro photosynthetic enzyme activity, gas exchange, and...[Show more]
Collections | ANU Research Publications |
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Date published: | 2012 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/53349 |
Source: | Plant Physiology |
DOI: | 10.1104/pp.112.203240 |
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01_Pengelly_Antisense_Reduction_of_2012.pdf | 258.61 kB | Adobe PDF | Request a copy |
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