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Antisense Reduction of NADP-Malic Enzyme in Flaveriabidentis Reduces Flow of CO 2 through the C 4 Cycle

Pengelly, Jasper; Tan, Jackie; Furbank, Robert Thomas; von Caemmerer, Susanne

Description

An antisense construct targeting the C4 isoform of NADP-malic enzyme (ME), the primary enzyme decarboxylating malate in bundle sheath cells to supply CO2 to Rubisco, was used to transform the dicot Flaveria bidentis. Transgenic plants (a-NADP-ME) exhibited a 34% to 75% reduction in NADP-ME activity relative to the wild type with no visible growth phenotype. We characterized the effect of reducing NADP-ME on photosynthesis by measuring in vitro photosynthetic enzyme activity, gas exchange, and...[Show more]

dc.contributor.authorPengelly, Jasper
dc.contributor.authorTan, Jackie
dc.contributor.authorFurbank, Robert Thomas
dc.contributor.authorvon Caemmerer, Susanne
dc.date.accessioned2015-12-10T22:25:08Z
dc.identifier.issn0032-0889
dc.identifier.urihttp://hdl.handle.net/1885/53349
dc.description.abstractAn antisense construct targeting the C4 isoform of NADP-malic enzyme (ME), the primary enzyme decarboxylating malate in bundle sheath cells to supply CO2 to Rubisco, was used to transform the dicot Flaveria bidentis. Transgenic plants (a-NADP-ME) exhibited a 34% to 75% reduction in NADP-ME activity relative to the wild type with no visible growth phenotype. We characterized the effect of reducing NADP-ME on photosynthesis by measuring in vitro photosynthetic enzyme activity, gas exchange, and real-time carbon isotope discrimination (Δ). In α-NADP-ME plants with less than 40% of wild-type NADP-ME activity, CO2 assimilation rates at high intercellular CO2 were significantly reduced, whereas the in vitro activities of both phosphoenolpyruvate carboxylase and Rubisco were increased. Δ measured concurrently with gas exchange in these plants showed a lower Δ and thus a lower calculated leakiness of CO2 (the ratio of CO2 leak rate from the bundle sheath to the rate of CO2 supply). Comparative measurements on antisense Rubisco small subunit F. bidentis plants showed the opposite effect of increased Δ and leakiness. We use these measurements to estimate the C4 cycle rate, bundle sheath leak rate, and bundle sheath CO2 concentration. The comparison of a-NADP-ME and antisense Rubisco small subunit demonstrates that the coordination of the C3 and C4 cycles that exist during environmental perturbations by light and CO2 can be disrupted through transgenic manipulations. Furthermore, our results suggest that the efficiency of the C4 pathway could potentially be improved through a reduction in C4 cycle activity or increased C3 cycle activity.
dc.publisherAmerican Society of Plant Biologists
dc.sourcePlant Physiology
dc.subjectKeywords: carbon; carbon dioxide; malate dehydrogenase; malate dehydrogenase (oxaloacetate decarboxylating) (NADP+); malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); phosphoenolpyruvate carboxylase; ribulosebisphosphate carboxylase; article; enzyme acti
dc.titleAntisense Reduction of NADP-Malic Enzyme in Flaveriabidentis Reduces Flow of CO 2 through the C 4 Cycle
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume160
dc.date.issued2012
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationu4956746xPUB271
local.type.statusPublished Version
local.contributor.affiliationPengelly, Jasper, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationTan, Jackie, National University of Singapore
local.contributor.affiliationFurbank, Robert Thomas, CSIRO Division of Plant Industry
local.contributor.affiliationvon Caemmerer, Susanne, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage1070
local.bibliographicCitation.lastpage1080
local.identifier.doi10.1104/pp.112.203240
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T11:27:45Z
local.identifier.scopusID2-s2.0-84867120217
local.identifier.thomsonID000309523800039
CollectionsANU Research Publications

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