Antisense Reduction of NADP-Malic Enzyme in Flaveriabidentis Reduces Flow of CO 2 through the C 4 Cycle
An antisense construct targeting the C4 isoform of NADP-malic enzyme (ME), the primary enzyme decarboxylating malate in bundle sheath cells to supply CO2 to Rubisco, was used to transform the dicot Flaveria bidentis. Transgenic plants (a-NADP-ME) exhibited a 34% to 75% reduction in NADP-ME activity relative to the wild type with no visible growth phenotype. We characterized the effect of reducing NADP-ME on photosynthesis by measuring in vitro photosynthetic enzyme activity, gas exchange, and...[Show more]
|Collections||ANU Research Publications|
|01_Pengelly_Antisense_Reduction_of_2012.pdf||258.61 kB||Adobe PDF||Request a copy|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.