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Antisense Reduction of NADP-Malic Enzyme in Flaveriabidentis Reduces Flow of CO 2 through the C 4 Cycle

Pengelly, Jasper; Tan, Jackie; Furbank, Robert Thomas; von Caemmerer, Susanne


An antisense construct targeting the C4 isoform of NADP-malic enzyme (ME), the primary enzyme decarboxylating malate in bundle sheath cells to supply CO2 to Rubisco, was used to transform the dicot Flaveria bidentis. Transgenic plants (a-NADP-ME) exhibited a 34% to 75% reduction in NADP-ME activity relative to the wild type with no visible growth phenotype. We characterized the effect of reducing NADP-ME on photosynthesis by measuring in vitro photosynthetic enzyme activity, gas exchange, and...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
Source: Plant Physiology
DOI: 10.1104/pp.112.203240


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