Pu, Fan; McKinney, Andrew; Stenhouse, Allen M; Suann, Craig J; McLeod, Malcolm
A study of the equine phase II metabolism of the anabolic agent boldenone is reported. Boldenone sulfate, boldenone glucuronide and their C17-epimers were synthesised as reference standards in our lab and a method was developed for their detection in a horse urine matrix. Solid phase extraction was used to purify the analytes, which were then detected by ion trap LC/MS. Negative and positive ionisation mode MS2 were used for the detection of sulfate and glucuronide conjugates, respectively....[Show more]
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