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A field-compatible method for measuring alternative respiratory pathway activities in vivo using stable O 2 isotopes

Kornfeld, Ari; Horton , Travis W; Yakir, Dan; Searle, Stephanie Y; Griffin, Kevin; Atkin, Owen; Subke, Jens-Arne; Turnbull, Matthew H

Description

Plants can alter rates of electron transport through the alternative oxidase (AOX) pathway in response to environmental cues, thus modulating respiratory efficiency, but the 18O discrimination method necessary for measuring electron partitioning in vivo has been restricted to laboratory settings. To overcome this limitation, we developed a field-compatible analytical method. Series of plant tissue subsamples were incubated in 12mL septum-capped vials for 0.5-4h before aliquots of incubation air...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
URI: http://hdl.handle.net/1885/52831
Source: Plant Cell and Environment
DOI: 10.1111/j.1365-3040.2012.02507.x

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