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A dipicolinic acid tag for rigid lanthanide tagging of proteins and paramagnetic NMR spectroscopy

Su, Xun-Cheng; Man, Bradley; Beeren, Sophie R.; Liang, Haobo; Simonsen, Shane; Schmitz, Christophe; Huber, Thomas; Messerle, Barbara Ann; Otting, Gottfried


A new lanthanide tag was designed for site-specific labeling of proteins with paramagnetic lanthanide ions. The tag, 4-mercaptomethyl-dipicolinic acid, binds lanthanide ions with nanomolar affinity, is readily attached to proteins via a disulfide bond, and avoids the problems of diastereomer formation associated with most of the conventional lanthanide tags. The high lanthanide affinity of the tag opens the possibility to measure residual dipolar couplings in a single sample containing a...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
Source: Journal of the American Chemical Society
DOI: 10.1021/ja803741f


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