Differential shrinkage of mesophyll cells in transpiring cotton leaves: implications for static and dynamic pools of water, and for water transport pathways
Download (3.79 MB)
-
Altmetric Citations
Canny, Martin; Wong, Suan Chin; Huang, Cheng; Miller, Celia
Description
Shrinkage of palisade cells during transpiration, previously measured for sclerophyllous leaves of Eucalyptus where cells shrank equally, was compared with shrinkage in thin mesophytic leaves of cotton (Gossypium hirsutum L.). Selected vapour pressure differences (e) from 0.6 to 2.7kPa were imposed during steady-state photosynthesis and transpiration. Leaves were then cryo-fixed and cryo-planed paradermally, and images obtained with a cryo-scanning electron microscope (CSEM). Diameters of...[Show more]
dc.contributor.author | Canny, Martin | |
---|---|---|
dc.contributor.author | Wong, Suan Chin | |
dc.contributor.author | Huang, Cheng | |
dc.contributor.author | Miller, Celia | |
dc.date.accessioned | 2015-12-10T22:19:09Z | |
dc.identifier.issn | 1445-4408 | |
dc.identifier.uri | http://hdl.handle.net/1885/51720 | |
dc.description.abstract | Shrinkage of palisade cells during transpiration, previously measured for sclerophyllous leaves of Eucalyptus where cells shrank equally, was compared with shrinkage in thin mesophytic leaves of cotton (Gossypium hirsutum L.). Selected vapour pressure differences (e) from 0.6 to 2.7kPa were imposed during steady-state photosynthesis and transpiration. Leaves were then cryo-fixed and cryo-planed paradermally, and images obtained with a cryo-scanning electron microscope (CSEM). Diameters of palisade 'cavity cells' within sub-stomatal cavities, and surrounding palisade 'matrix cells' were measured on CSEM images. Cavity and spongy mesophyll cells shrank progressively down to Δe=2.7kPa, while matrix cells remained at the same diameter at all Δe. Diameters were also measured of cavity and matrix cells quasi-equilibrated with relative humidities (RHs) from 100% to 86%. In leaves quasi-equilibrated with 95% RH, the cavity cells shrank so much as to be almost unmeasurable, while matrix cells shrank by only 6%. These data suggest that there are two distinct pools of water in cotton leaves: cavity plus spongy mesophyll cells (two-thirds of leaf volume) which easily lose water; and matrix cells (one-third of leaf volume), which retain turgor down to relative water loss=0.4, providing structural rigidity to prevent wilting. This phenomenon is probably widespread among mesophytic leaves. | |
dc.publisher | CSIRO Publishing | |
dc.source | Functional Plant Biology | |
dc.subject | Keywords: cotton; cryopreservation; cytology; diameter; evaporation; leaf; photosynthesis; physiological response; relative humidity; rigidity; scanning electron microscopy; shrub; steady-state equilibrium; stomatal conductance; vapor pressure; wilting cell interconnections; relative water loss; sites of evaporation; stomatal cavities; transpiration pathway; wilting resistance | |
dc.title | Differential shrinkage of mesophyll cells in transpiring cotton leaves: implications for static and dynamic pools of water, and for water transport pathways | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 39 | |
dc.date.issued | 2012 | |
local.identifier.absfor | 060705 - Plant Physiology | |
local.identifier.absfor | 060112 - Structural Biology (incl. Macromolecular Modelling) | |
local.identifier.ariespublication | u4956746xPUB230 | |
local.type.status | Published Version | |
local.contributor.affiliation | Canny, Martin, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Wong, Suan Chin, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Huang, Cheng, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Miller, Celia, CSIRO Division of Plant Industry | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 91 | |
local.bibliographicCitation.lastpage | 102 | |
local.identifier.doi | 10.1071/FP11172 | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2016-02-24T11:27:25Z | |
local.identifier.scopusID | 2-s2.0-84863115675 | |
local.identifier.thomsonID | 000300319200002 | |
Collections | ANU Research Publications |
Download
File | Description | Size | Format | Image |
---|---|---|---|---|
01_Canny_Differential_shrinkage_of_2012.pdf | 3.79 MB | Adobe PDF | ||
02_Canny_Differential_shrinkage_of_2012.pdf | 3.81 MB | Adobe PDF |
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.
Updated: 17 November 2022/ Responsible Officer: University Librarian/ Page Contact: Library Systems & Web Coordinator