Development of fluorescent in situ hybridisation for Cryptosporidium detection reveals zoonotic and anthrioponotic transmission of sporadic cryptosporidiosis in Sydney
Cryptosporidium, is the most common non-viral cause of diarrhea worldwide. Of the 5 described species that contribute to the majority of human infections, C. parvum is of major interest due to its zoonotic potential. A species-specific fluorescence in situ hybridisation probe was designed to the variable region in the small subunit of the 18S rRNA of C. parvum and labeled with Cy3. Probe specificity was validated against a panel of 7 other Cryptosporidium spp. before it was applied to 33 human...[Show more]
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|Source:||Journal of Microbiological Methods|
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