Imaging pluripotent cell migration in Drosophila
-
Altmetric Citations
Murray, Michael; Saint, Robert
Description
Drosophila melanogaster offers a powerful system for the analysis of cell migration. In the embryo, -pluripotent cells of the mesodermal and endodermal primordia undergo epithelial-mesenchymal transitions and cell migration, while primordial germ cells migrate through an endodermal barrier to form the gonads. Visualisation of these migrations has traditionally been achieved by staining fixed embryos at different developmental stages or through live imaging of cells using tissue-specific...[Show more]
dc.contributor.author | Murray, Michael | |
---|---|---|
dc.contributor.author | Saint, Robert | |
dc.date.accessioned | 2015-12-10T22:18:19Z | |
dc.identifier.isbn | 9781617791444 | |
dc.identifier.uri | http://hdl.handle.net/1885/51369 | |
dc.description.abstract | Drosophila melanogaster offers a powerful system for the analysis of cell migration. In the embryo, -pluripotent cells of the mesodermal and endodermal primordia undergo epithelial-mesenchymal transitions and cell migration, while primordial germ cells migrate through an endodermal barrier to form the gonads. Visualisation of these migrations has traditionally been achieved by staining fixed embryos at different developmental stages or through live imaging of cells using tissue-specific expression of marker fluorescent proteins. More recently, photoactivatable fluorescence proteins have allowed the labelling of small groups of cells or single cells so that their migratory patterns and fate can be followed. By fusing the photoactivatable fluorescent protein to proteins that mark different subcellular components, it is now possible to visualise different aspects of the cells as they migrate. Here, we review previous studies of the migration of pluripotent embryonic cells and describe, in detail, methods for visualising these cells. | |
dc.publisher | Humana Press Inc. | |
dc.relation.ispartof | Stem Cell Migration: Methods and Protocols | |
dc.subject | Keywords: fixative; green fluorescent protein; animal; animal embryo; article; cell motion; chemistry; confocal microscopy; cytology; Drosophila melanogaster; epithelial mesenchymal transition; fluorescence; gene expression regulation; genetics; germ cell; gonad; i | |
dc.title | Imaging pluripotent cell migration in Drosophila | |
dc.type | Book chapter | |
local.description.notes | Imported from ARIES | |
dc.date.issued | 2011 | |
local.identifier.absfor | 060103 - Cell Development, Proliferation and Death | |
local.identifier.absfor | 060106 - Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) | |
local.identifier.ariespublication | u8611701xPUB222 | |
local.type.status | Published Version | |
local.contributor.affiliation | Murray, Michael, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Saint, Robert, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 169 | |
local.bibliographicCitation.lastpage | 184 | |
local.identifier.doi | 10.1007/978-1-61779-145-1_12 | |
local.identifier.absseo | 929999 - Health not elsewhere classified | |
dc.date.updated | 2016-02-24T11:41:37Z | |
local.bibliographicCitation.placeofpublication | New York | |
local.identifier.scopusID | 2-s2.0-80052609713 | |
Collections | ANU Research Publications |
Download
File | Description | Size | Format | Image |
---|---|---|---|---|
01_Murray_Imaging_pluripotent_cell_2011.pdf | 554.11 kB | Adobe PDF | Request a copy | |
02_Murray_Imaging_pluripotent_cell_2011.pdf | 109.61 kB | Adobe PDF | Request a copy |
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.
Updated: 17 November 2022/ Responsible Officer: University Librarian/ Page Contact: Library Systems & Web Coordinator