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Cell-free protein synthesis for analysis by NMR spectroscopy

Apponyi, Margit; Ozawa, Kiyoshi; Dixon, Nicholas; Otting, Gottfried

Description

Cell-free protein synthesis offers fast and inexpensive access to selectively isotope labeled proteins that can be measured by NMR spectroscopy in the presence of all the unlabeled proteins in the reaction mixture. No chromatographic purification is required. Using an extract from Escherichia coli in a simple dialysis system, the target protein can be prepared at a typical concentration of about 1 mg/ml, which is sufficient for subsequent analysis by NMR. This chapter describes in detail the...[Show more]

dc.contributor.authorApponyi, Margit
dc.contributor.authorOzawa, Kiyoshi
dc.contributor.authorDixon, Nicholas
dc.contributor.authorOtting, Gottfried
dc.date.accessioned2015-12-10T22:16:41Z
dc.identifier.isbn9781588298096
dc.identifier.urihttp://hdl.handle.net/1885/51064
dc.description.abstractCell-free protein synthesis offers fast and inexpensive access to selectively isotope labeled proteins that can be measured by NMR spectroscopy in the presence of all the unlabeled proteins in the reaction mixture. No chromatographic purification is required. Using an extract from Escherichia coli in a simple dialysis system, the target protein can be prepared at a typical concentration of about 1 mg/ml, which is sufficient for subsequent analysis by NMR. This chapter describes in detail the protocol used in the authors' laboratory.
dc.publisherHumana Press Inc.
dc.relation.ispartofStructural Proteomics: High Throughput Methods
dc.relation.isversionof1st Edition
dc.subjectKeywords: Escherichia coli protein; nitrogen; article; cell free system; chemistry; Escherichia coli; genetics; isotope labeling; metabolism; methodology; nuclear magnetic resonance spectroscopy; protein synthesis; Cell-Free System; Escherichia coli; Escherichia co
dc.titleCell-free protein synthesis for analysis by NMR spectroscopy
dc.typeBook chapter
local.description.notesImported from ARIES
dc.date.issued2008
local.identifier.absfor060113 - Synthetic Biology
local.identifier.ariespublicationu4005981xPUB216
local.type.statusPublished Version
local.contributor.affiliationApponyi, Margit, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOzawa, Kiyoshi, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationDixon, Nicholas, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOtting, Gottfried, College of Physical and Mathematical Sciences, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage257
local.bibliographicCitation.lastpage268
dc.date.updated2015-12-09T08:26:37Z
local.bibliographicCitation.placeofpublicationTotowa, NJ, USA
local.identifier.scopusID2-s2.0-49649111497
CollectionsANU Research Publications

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