Skip navigation
Skip navigation

Cell-free protein synthesis for analysis by NMR spectroscopy

Apponyi, Margit; Ozawa, Kiyoshi; Dixon, Nicholas; Otting, Gottfried


Cell-free protein synthesis offers fast and inexpensive access to selectively isotope labeled proteins that can be measured by NMR spectroscopy in the presence of all the unlabeled proteins in the reaction mixture. No chromatographic purification is required. Using an extract from Escherichia coli in a simple dialysis system, the target protein can be prepared at a typical concentration of about 1 mg/ml, which is sufficient for subsequent analysis by NMR. This chapter describes in detail the...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Book chapter


File Description SizeFormat Image
01_Apponyi_Cell-free_protein_synthesis_2008.pdf979.19 kBAdobe PDF    Request a copy

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  20 July 2017/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator