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Cell-free protein synthesis for analysis by NMR spectroscopy

Apponyi, Margit; Ozawa, Kiyoshi; Dixon, Nicholas; Otting, Gottfried


Cell-free protein synthesis offers fast and inexpensive access to selectively isotope labeled proteins that can be measured by NMR spectroscopy in the presence of all the unlabeled proteins in the reaction mixture. No chromatographic purification is required. Using an extract from Escherichia coli in a simple dialysis system, the target protein can be prepared at a typical concentration of about 1 mg/ml, which is sufficient for subsequent analysis by NMR. This chapter describes in detail the...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Book chapter
Book Title: Structural Proteomics: High Throughput Methods


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