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Antigen-Epitope Retrieval To Facilitate Proteomic Analysis of Formalin-Fixed Archival Brain Tissue

Rahimi, Ahmed (Farid); Shepherd, Claire E; Halliday, Glenda M; Geczy, Carolyn; Raftery, Mark


Formalin is a routine fixative facilitating tissue preservation and histopathology. Proteomic techniques require freshly frozen specimens, which are often difficult to procure, and methods facilitating proteomic analysis of archival formalin-fixed brain tissue are lacking. We employed antigen-epitope-retrieval principles to facilitate proteomic analysis of brain tissue that had been fixed and stored in formalin for 3-7 years. Twenty-micrometer-thick cryopreserved OCT-embedded sections from...[Show more]

CollectionsANU Research Publications
Date published: 2006
Type: Journal article
Source: Analytical Chemistry
DOI: 10.1021/ac060294s


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