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Lanthanide-binding peptides for NMR measurements of residual dipolar couplings and paramagnetic effects from multiple angles

Su, Xun-Cheng; McAndrew, Kerry; Huber, Thomas; Otting, Gottfried

Description

Lanthanide-binding peptide tags (LBTs) containing a single cysteine residue can be attached to proteins via a disulfide bond, presenting a flexible means of tagging proteins site-specifically with a lanthanide ion. Here we show that cysteine residues placed in different positions of the LBT can be used to expose the protein to different orientations of the magnetic susceptibility anisotropy (Δχ) tensor and to generate different molecular alignments in a magnetic field. Δχ tensors determined by...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
URI: http://hdl.handle.net/1885/50293
Source: Journal of the American Chemical Society
DOI: 10.1021/ja076564l

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