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Characterization of gibberellin receptor mutants of barley ( Hordeum vulgare L.)

Chandler, Peter; Harding, Carol A; Ashton, Anthony; Mulcair, Mark; Dixon, Nicholas; Mander, Lewis

Description

The sequence of Gid1 (a gene for a gibberellin (GA) receptor from rice) was used to identify a putative orthologue from barley. This was expressed in E. coli, and produced a protein that was able to bind GA in vitro with both structural specificity and saturability. Its potential role in GA responses was investigated using barley mutants with reduced GA sensitivity (gse1 mutants). Sixteen different gse1 mutants each carried a unique nucleotide substitution in this sequence. In all but one case,...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
URI: http://hdl.handle.net/1885/50239
Source: Molecular Plant
DOI: 10.1093/mp/ssn002

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