No evidence from FTIR Difference Spectroscopy That Glutamate-189 of the D1 Polypeptide Ligates a Mn Ion That Undergoes Oxidation during the S0 to S1, S1 to S2, or S2 to S3 Transitions in Photosystem II
In the recent X-ray crystallographic structural models of photosystem II, Glu189 of the D1 polypeptide is assigned as a ligand of the oxygen-evolving Mn4 cluster. To determine if D1-Glu189 ligates a Mn ion that undergoes oxidation during one or more of the S0 → S 1, S1 → S2, and S2 → S3 transitions, the FTIR difference spectra of the individual S-state transitions in D1-E189Q and D1-E189R mutant PSII particles from the cyanobacterium Synechocystis sp. PCC 6803 were compared with those in...[Show more]
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