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Regulation of S100A8 by Glucocorticoids

Hsu, Kenneth; Passey, Robert J; Endoh, Yasumi; Rahimi, Ahmed (Farid); Youssef, Peter; Yen, Tina; Geczy, Carolyn

Description

S100A8 (A8) has roles in inflammation, differentiation and development and is associated with oxidative defense. Murine A8 (mA8) is up-regulated in macrophages, fibroblasts, and microvascular endothelial cells by LPS. Glucocorticoids (GCs) amplified LPS-induced mA8 in these cells. Relative to stimulation by LPS, GCs increased mA8 gene transcription and mRNA half-life. Enhancement required new protein synthesis, IL-10 and products of the cyclooxygenase-2 pathway, and both ERK1/2 and p38 MAPK....[Show more]

dc.contributor.authorHsu, Kenneth
dc.contributor.authorPassey, Robert J
dc.contributor.authorEndoh, Yasumi
dc.contributor.authorRahimi, Ahmed (Farid)
dc.contributor.authorYoussef, Peter
dc.contributor.authorYen, Tina
dc.contributor.authorGeczy, Carolyn
dc.date.accessioned2015-12-10T22:14:00Z
dc.identifier.issn0022-1767
dc.identifier.urihttp://hdl.handle.net/1885/50051
dc.description.abstractS100A8 (A8) has roles in inflammation, differentiation and development and is associated with oxidative defense. Murine A8 (mA8) is up-regulated in macrophages, fibroblasts, and microvascular endothelial cells by LPS. Glucocorticoids (GCs) amplified LPS-induced mA8 in these cells. Relative to stimulation by LPS, GCs increased mA8 gene transcription and mRNA half-life. Enhancement required new protein synthesis, IL-10 and products of the cyclooxygenase-2 pathway, and both ERK1/2 and p38 MAPK. Protein kinase A positively and protein kinase C negatively regulated this process. Promoter analysis indicated element(s) essential for LPS and dexamethasone enhancement colocated within the region -178 to 0 bp. In the absence of glucocorticoid response elements, NF1 motif at -58 is a candidate for mediation of enhancement. Gel shift analysis detected no differences between LPS- and LPS/dexamethasone-treated complexes within this region. GCs increased constitutive levels of A8 and S100A9 (A9) mRNA in human monocytes. The synovial membrane of rheumatoid patients treated with high dose i.v. methylprednisolone contained higher numbers of A8/A9-positive macrophages than pre- or posttreatment samples. Results support the proposal that A8 has anti-inflammatory properties that may be independent of hetero-complex formation with A9 and may also enable localized defense in the absence of overriding deleterious host responses.
dc.publisherAmerican Association of Immunologists
dc.sourceJournal of Immunology
dc.source.urihttp://www.jimmunol.org/content/174/4/2318.full.html#ref-list-1
dc.subjectKeywords: cyclic AMP dependent protein kinase; cyclooxygenase 2; dexamethasone; glucocorticoid; interleukin 10; lipopolysaccharide; messenger RNA; methylprednisolone; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein
dc.titleRegulation of S100A8 by Glucocorticoids
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume174
dc.date.issued2005
local.identifier.absfor110316 - Pathology
local.identifier.absfor110799 - Immunology not elsewhere classified
local.identifier.ariespublicationu8611701xPUB196
local.type.statusPublished Version
local.contributor.affiliationHsu, Kenneth, University of New South Wales
local.contributor.affiliationPassey, Robert J, University of New South Wales
local.contributor.affiliationEndoh, Yasumi, University of New South Wales
local.contributor.affiliationRahimi, Ahmed (Farid), College of Medicine, Biology and Environment, ANU
local.contributor.affiliationYoussef, Peter, Royal Prince Alfred Hospital
local.contributor.affiliationYen, Tina, University of New South Wales
local.contributor.affiliationGeczy, Carolyn, University of New South Wales
local.description.embargo2037-12-31
local.bibliographicCitation.startpage2318
local.bibliographicCitation.lastpage2326
local.identifier.absseo920108 - Immune System and Allergy
dc.date.updated2015-12-09T08:02:35Z
local.identifier.scopusID2-s2.0-13544271152
CollectionsANU Research Publications

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