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Isoleucine 309 acts as a C 4 catalytic switch that increases ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) carboxylation rate in Flaveria

Whitney, Spencer; Sharwood, Robert; Orr, Douglas; White, Sarah; Alonso, Hernan; Galmes, Jeroni

Description

Improving global yields of important agricultural crops is a complex challenge. Enhancing yield and resource use by engineering improvements to photosynthetic carbon assimilation is one potential solution. During the last 40 million years C4 photosynthesis has evolved multiple times, enabling plants to evade the catalytic inadequacies of the CO2-fixing enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco). Compared with their C3 ancestors, C4 plants combine a faster rubisco with a...[Show more]

dc.contributor.authorWhitney, Spencer
dc.contributor.authorSharwood, Robert
dc.contributor.authorOrr, Douglas
dc.contributor.authorWhite, Sarah
dc.contributor.authorAlonso, Hernan
dc.contributor.authorGalmes, Jeroni
dc.date.accessioned2015-12-10T22:13:35Z
dc.identifier.issn0027-8424
dc.identifier.urihttp://hdl.handle.net/1885/49804
dc.description.abstractImproving global yields of important agricultural crops is a complex challenge. Enhancing yield and resource use by engineering improvements to photosynthetic carbon assimilation is one potential solution. During the last 40 million years C4 photosynthesis has evolved multiple times, enabling plants to evade the catalytic inadequacies of the CO2-fixing enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco). Compared with their C3 ancestors, C4 plants combine a faster rubisco with a biochemical CO2- concentrating mechanism, enabling more efficient use of water and nitrogen and enhanced yield. Here we show the versatility of plastome manipulation in tobacco for identifying sequences in C4-rubisco that can be transplanted into C3-rubisco to improve carboxylation rate (VC). Using transplastomic tobacco lines expressing native and mutated rubisco large subunits (L-subunits) from Flaveria pringlei (C3), Flaveria floridana (C3-C4), and Flaveria bidentis (C4), we reveal that Met-309-Ile substitutions in the L-subunit act as a catalytic switch between C4 (309Ile; faster VC, lower CO2 affinity) and C3 (309Met; slower VC, higher CO2 affinity) catalysis. Application of this transplastomic system permits further identification of other structural solutions selected by nature that can increase rubisco VC in C3 crops. Coengineering a catalytically faster C3 rubisco and a CO2-concentrating mechanism within C3 crop species could enhance their efficiency in resource use and yield.
dc.publisherNational Academy of Sciences (USA)
dc.rightsAuthor/s retain copyright
dc.sourcePNAS - Proceedings of the National Academy of Sciences of the United States of America
dc.subjectKeywords: carbon dioxide; isoleucine; nitrogen; ribulosebisphosphate carboxylase; water; amino acid sequence; amino acid substitution; article; carbon fixation; carboxylation; chloroplast; controlled study; Flaveria; Flaveria bidentis; Flaveria floridana; Flaveria Chloroplast transformation; CO 2 assimilation; Gas exchange; rbcL mutagenesis
dc.titleIsoleucine 309 acts as a C 4 catalytic switch that increases ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) carboxylation rate in Flaveria<i/>
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume108
dc.date.issued2011
local.identifier.absfor060107 - Enzymes
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationu4956746xPUB192
local.type.statusPublished Version
local.contributor.affiliationWhitney, Spencer, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSharwood, Robert, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationOrr, Douglas, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWhite, Sarah, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationAlonso, Hernan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGalmes, Jeroni, MediterrĂ nies Universitat de les Illes Balears
local.bibliographicCitation.issue35
local.bibliographicCitation.startpage14688
local.bibliographicCitation.lastpage14693
local.identifier.doi10.1073/pnas.1109503108
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T11:27:05Z
local.identifier.scopusID2-s2.0-80052276565
local.identifier.thomsonID000294425900064
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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