Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, Sf6, of Shigella flexneri
The modification of the LPS O-antigen, seen in the diverse serotypes of Shigella flexneri is brought about by the glucosyltransferases (Gtr) and the O-acetyltransferase (Oac). In this study, we establish the membrane topology of Oac using the dual reporter PhoA-LacZα. We have determined that Oac is an integral membrane protein with 10 transmembrane regions. The hydrophilic N- and C-termini are oriented in the cytoplasm. Functionally important cytoplasmic and periplasmic loops have also been...[Show more]
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|Source:||Biochemical and Biophysical Research Communications|
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