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Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, Sf6, of Shigella flexneri

Thanweer, Farzaana; Tahiliani, Vikas; Korres, Haralambos; Verma, Naresh


The modification of the LPS O-antigen, seen in the diverse serotypes of Shigella flexneri is brought about by the glucosyltransferases (Gtr) and the O-acetyltransferase (Oac). In this study, we establish the membrane topology of Oac using the dual reporter PhoA-LacZ╬▒. We have determined that Oac is an integral membrane protein with 10 transmembrane regions. The hydrophilic N- and C-termini are oriented in the cytoplasm. Functionally important cytoplasmic and periplasmic loops have also been...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
Source: Biochemical and Biophysical Research Communications
DOI: 10.1016/j.bbrc.2008.08.069


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