Anas, Mohamed-Kheir Idris; Lee, Martin B; Zhou, Chenxi; Hammer, Mary-Anne; Slow, Sandy; Karmouch, Jennifer; Liu, X. Johne; Broer, Stefan; Lever, Michael; Baltz, Jay M
Betaine (N,N,N-trimethylglycine) added to culture media is known to substantially improve the development of preimplantation mouse embryos in vitro, and to be imported into 1-cell embryos by a transporter that also accepts proline. Here, we found that the betaine/proline transporter is active in preimplantation mouse embryos only for a short period of development, between the 1- and 2-cell stages. Betaine/proline transport was activated after fertilization, beginning ∼4 hours post-egg...[Show more]
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