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Anti-inflammatory activity, cytotoxicity and active compounds of Tinospora smilacina

Li, Rachel; Myers, S P; Leach, D N; Lin, G D; Leach, G J; Brushett, D J; Waterman, P G

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Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2...[Show more]

dc.contributor.authorLi, Rachel
dc.contributor.authorMyers, S P
dc.contributor.authorLeach, D N
dc.contributor.authorLin, G D
dc.contributor.authorLeach, G J
dc.contributor.authorBrushett, D J
dc.contributor.authorWaterman, P G
dc.date.accessioned2015-12-10T21:57:42Z
dc.identifier.issn0951-418X
dc.identifier.urihttp://hdl.handle.net/1885/39895
dc.description.abstractTinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and phospholipase A2 (PA2). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX-1, COX-2, 5-LO and PA2 with the IC50 values of 63.5, 81.2, 92.1 and 30.5 μg/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP-based luminescence assay and the results showed no cytotoxic effect on cell lines of skin fibroblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL-60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti- inflammatory activity is due to triterpene-fatty acid esters and free fatty acids.
dc.publisherJohn Wiley & Sons Inc
dc.sourcePhytotherapy Research
dc.subjectKeywords: acetylsalicylic acid; arachidonate 5 lipoxygenase; cyclooxygenase 1; cyclooxygenase 2; fatty acid; fatty acid ester; indometacin; phospholipase A2; plant extract; tinospora smilacina extract; unclassified drug; antiinflammatory activity; article; Australi 5-lipoxygenase; Anti-inflammatory activity; Cycloxygenase-1 and -2; Cytotoxicity; LC-MS; NMR; Tinospora smilacina
dc.titleAnti-inflammatory activity, cytotoxicity and active compounds of Tinospora smilacina
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume18
dc.date.issued2004
local.identifier.absfor111599 - Pharmacology and Pharmaceutical Sciences not elsewhere classified
local.identifier.ariespublicationu4105084xPUB185
local.type.statusPublished Version
local.contributor.affiliationLi, Rachel, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMyers, S P, University of Queensland
local.contributor.affiliationLeach, D N, University of Queensland
local.contributor.affiliationLin, G D, University of Queensland
local.contributor.affiliationLeach, G J, University of Queensland
local.contributor.affiliationBrushett, D J, University of Hawaii
local.contributor.affiliationWaterman, P G, University of Queensland
local.description.embargo2037-12-31
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage78
local.bibliographicCitation.lastpage83
local.identifier.doi10.1002/ptr.1373
dc.date.updated2015-12-09T07:47:49Z
local.identifier.scopusID2-s2.0-0842291442
CollectionsANU Research Publications

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