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In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity

Quah, Ben; Ni, Keping; O'Neill, Helen

Description

The study of dendritic cells (DC) has been hampered by the difficulty of isolating rare cells for analysis of their phenotype and function. Interpretation of the DC lineage has been largely influenced by studies on cell populations which can be readily isolated and amplified in the presence of cytokines. Long term cultures (LTC) from murine spleen have been shown to support continuous in vitro hematopoiesis of DC dependent on interaction with a stromal cell monolayer. LTC-DC represent a single,...[Show more]

dc.contributor.authorQuah, Ben
dc.contributor.authorNi, Keping
dc.contributor.authorO'Neill, Helen
dc.date.accessioned2015-12-10T21:57:34Z
dc.identifier.issn0953-8178
dc.identifier.urihttp://hdl.handle.net/1885/39835
dc.description.abstractThe study of dendritic cells (DC) has been hampered by the difficulty of isolating rare cells for analysis of their phenotype and function. Interpretation of the DC lineage has been largely influenced by studies on cell populations which can be readily isolated and amplified in the presence of cytokines. Long term cultures (LTC) from murine spleen have been shown to support continuous in vitro hematopoiesis of DC dependent on interaction with a stromal cell monolayer. LTC-DC represent a single, stable class of DC derived by constant turnover of spleen DC progenitors maintained within stroma. They represent a resident DC population in spleen. The functional characteristics of LTC-DC have been studied in terms of capacity to stimulate T cells and response to activation by environmental stimuli. LTC-DC have many morphological, phenotypic and functional properties reflecting an immature or partially mature, marginal zone-like CD4-CD8- splenic DC subset. They are highly endocytic and can process and present protein antigen to naive hen egg lysozyme (HEL)-specific MHC-II-restricted TCR-Tg CD4+ T cells. They do not, however, induce T cell proliferation in a mixed lymphocyte reaction. LTC-DC do not respond in a typical fashion to common DC activators like LPS and CD40L. They upregulate MHC-I and CD80/CD86 but not MHC-II and CD40. They reflect an endogenous, immature DC subset in spleen with properties distinct from immature DC located in peripheral tissues.
dc.publisherOxford University Press
dc.sourceInternational Immunology
dc.subjectKeywords: B7 antigen; CD4 antigen; CD40 ligand; CD8 antigen; CD86 antigen; cytokine; lipopolysaccharide; lysozyme; major histocompatibility antigen class 1; major histocompatibility antigen class 2; T lymphocyte receptor; animal cell; antigen presentation; article; Antigen presentation; Dendritic cells; Hematopoiesis; T cells
dc.titleIn vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume16
dc.date.issued2004
local.identifier.absfor110704 - Cellular Immunology
local.identifier.ariespublicationu4167262xPUB184
local.type.statusPublished Version
local.contributor.affiliationQuah, Ben, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationNi, Keping, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationO'Neill, Helen, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue4
local.bibliographicCitation.startpage567
local.bibliographicCitation.lastpage577
local.identifier.doi10.1093/intimm/dxh060
dc.date.updated2015-12-09T07:46:35Z
local.identifier.scopusID2-s2.0-1842832883
CollectionsANU Research Publications

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