Skip navigation
Skip navigation

Strategies for measurements of pseudocontact shifts in protein NMR spectroscopy

John, Michael; Otting, Gottfried


Paramagnetic metal ions bound to proteins generate a dipolar field that can be accurately probed by pseudocontact shifts (PCS) displayed by the protein's nuclear spins. PCS are highly useful for determining the coordinates of individual spins in the molecule and for rapid structure determinations of entire protein-protein and protein-ligand complexes. However, PCS measurements require reliable resonance assignments for the molecule in its paramagnetic state and in a diamagnetic reference state....[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: ChemPhysChem
DOI: 10.1002/cphc.200700510


File Description SizeFormat Image
01_John_Strategies_for_measurements_of_2007.pdf641.23 kBAdobe PDFThumbnail

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator