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The Tomato NBARC-LRR Protein Prf Interacts with Pto Kinase in Vivo to Regulate Specific Plant Immunity

Mucyn, Tatiana; Clemente, Alfonso; Andriotis, Vasilios M E; Balmuth, Alexi L; Oldroyd, Giles E D; Staskawicz, B J; Rathjen, John

Description

Immunity in tomato (Solanum lycopersicum) to Pseudomonas syringae bacteria expressing the effector proteins AvrPto and AvrPtoB requires both Pto kinase and the NBARC-LRR (for nucleotide binding domain shared by Apaf-1, certain R gene products, and CED-4 fused to C-terminal leucine-rich repeats) protein Prf. Pto plays a direct role in effector recognition within the host cytoplasm, but the role of Prf is unknown. We show that Pto and Prf are coincident in the signal transduction pathway that...[Show more]

dc.contributor.authorMucyn, Tatiana
dc.contributor.authorClemente, Alfonso
dc.contributor.authorAndriotis, Vasilios M E
dc.contributor.authorBalmuth, Alexi L
dc.contributor.authorOldroyd, Giles E D
dc.contributor.authorStaskawicz, B J
dc.contributor.authorRathjen, John
dc.date.accessioned2015-12-10T21:53:37Z
dc.identifier.issn1040-4651
dc.identifier.urihttp://hdl.handle.net/1885/38580
dc.description.abstractImmunity in tomato (Solanum lycopersicum) to Pseudomonas syringae bacteria expressing the effector proteins AvrPto and AvrPtoB requires both Pto kinase and the NBARC-LRR (for nucleotide binding domain shared by Apaf-1, certain R gene products, and CED-4 fused to C-terminal leucine-rich repeats) protein Prf. Pto plays a direct role in effector recognition within the host cytoplasm, but the role of Prf is unknown. We show that Pto and Prf are coincident in the signal transduction pathway that controls ligand-independent signaling. Pto and Prf associate in a coregulatory interaction that requires Pto kinase activity and N-myristoylation for signaling. Pto interacts with a unique Prf N-terminal domain outside of the NBARC-LRR domain and resides in a high molecular weight recognition complex dependent on the presence of Prf. In this complex, both Pto and Prf contribute to specific recognition of AvrPtoB. The data suggest that the role of Pto is confined to the regulation of Prf and that the bacterial effectors have evolved to target this coregulatory molecular switch.
dc.publisherAmerican Society of Plant Biologists
dc.sourceThe Plant Cell
dc.subjectKeywords: Bacteria; Genetic engineering; Immunology; Plants (botany); Coregulatory interaction; Effector recognition; Host cytoplasm; Pseudomonas syringae bacteria; Proteins; Prf protein, Lycopersicon esculentum; primer DNA; protein serine threonine kinase; Pto pro
dc.titleThe Tomato NBARC-LRR Protein Prf Interacts with Pto Kinase in Vivo to Regulate Specific Plant Immunity
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume18
dc.date.issued2006
local.identifier.absfor060704 - Plant Pathology
local.identifier.ariespublicationu4956746xPUB163
local.type.statusPublished Version
local.contributor.affiliationMucyn, Tatiana, The Sainsbury Laboratory
local.contributor.affiliationClemente, Alfonso, The Sainsbury Laboratory
local.contributor.affiliationAndriotis, Vasilios M E , The Sainsbury Laboratory
local.contributor.affiliationBalmuth, Alexi L, The Sainsbury Laboratory
local.contributor.affiliationOldroyd, Giles E D , University of California
local.contributor.affiliationStaskawicz, B J , Univeristy of California
local.contributor.affiliationRathjen, John, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage2792
local.bibliographicCitation.lastpage2806
local.identifier.doi10.1105/tpc.106.044016
local.identifier.absseo820215 - Vegetables
dc.date.updated2015-12-09T07:19:12Z
local.identifier.scopusID2-s2.0-33750988221
CollectionsANU Research Publications

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