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Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana

Jones, Alexandra; MacLean , Daniel; Studholme , David J; Serna -Sanz, Antonio; Andreasson , Erik; Rathjen, John; Peck, Scott C

Description

Phosphorylation is a ubiquitous regulatory mechanism, that governs the activity, subcellular localisation and molecular interactions of proteins. To identify a broad range of nuclear phosphoproteins from Arabidopsis thaliana, we enriched for nuclei from suspension cell cultures and seedlings before extensive fractionation and identification of phosphopeptides by mass spectrometry. We identified 416 phosphopeptides from 345 proteins with high confidence. Our data show that sub-cellular...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
URI: http://hdl.handle.net/1885/38390
Source: Journal of Proteomics
DOI: 10.1016/j.jprot.2009.02.004

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