Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana
Jones, Alexandra; MacLean , Daniel; Studholme , David J; Serna -Sanz, Antonio; Andreasson , Erik; Rathjen, John; Peck, Scott C
Phosphorylation is a ubiquitous regulatory mechanism, that governs the activity, subcellular localisation and molecular interactions of proteins. To identify a broad range of nuclear phosphoproteins from Arabidopsis thaliana, we enriched for nuclei from suspension cell cultures and seedlings before extensive fractionation and identification of phosphopeptides by mass spectrometry. We identified 416 phosphopeptides from 345 proteins with high confidence. Our data show that sub-cellular...[Show more]
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|Source:||Journal of Proteomics|
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