Watt, Stephen J; Sheil, Margaret; Beck, Jennifer; Prosselkov, Pavel; Otting, Gottfried; Dixon, Nicholas
Changes in protein conformation are thought to alter charge state distributions observed in electrospray ionization mass spectra (ESI-MS) of proteins. In most cases, this has been demonstrated by unfolding proteins through acidification of the solution. This methodology changes the properties of the solvent so that changes in the ESI-MS charge envelopes from conformational changes are difficult to separate from the effects of changing solvent on the ionization process. A novel strategy is...[Show more]
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