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Effect of protein stabilization on charge state distribution in positive- and negative-ion electrospray ionization mass spectra

Watt, Stephen J; Sheil, Margaret; Beck, Jennifer; Prosselkov, Pavel; Dixon, Nicholas; Otting, Gottfried


Changes in protein conformation are thought to alter charge state distributions observed in electrospray ionization mass spectra (ESI-MS) of proteins. In most cases, this has been demonstrated by unfolding proteins through acidification of the solution. This methodology changes the properties of the solvent so that changes in the ESI-MS charge envelopes from conformational changes are difficult to separate from the effects of changing solvent on the ionization process. A novel strategy is...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: Journal of the American Society for Mass Spectrometry
DOI: 10.1016/j.jasms.2007.06.004


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