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Using directed evolution to improve the solubility of the C- terminal domain of Escherichia coli aminopeptidase P: Implications for metal binding and protein stability

Liu, Jian-Wei; Hadler, Kieran S; Schenk, Gerhard; Ollis, David


There have been many approaches to solving problems associated with protein solubility. This article describes the application of directed evolution to improving the solubility of the C-terminal metal-binding domain of aminopeptidase P from Escherichia coli. During the course of experiments, the domain boundary and sequence were allowed to vary. It was found that extending the domain boundary resulted in aggregation with little improvement in solubility, whereas two changes to the sequence of...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: The FEBS Journal
DOI: 10.1111/j.1742-4658.2007.06022.x


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