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Gene signature of Stromal Cells which support Dendritic Cell Development

Despars, Genevieve; Periasamy, Pravin; Tan, Jonathan; Abbey, Janice; O'Neill, Terence; O'Neill, Helen

Description

Spleen stromal cells are critical determinants of dendritic cell (DC) development in spleen. The spleen stromal line, namely STX3, supports DC differentiation in vitro from overlaid bone marrow cells while the lymph node stromal line, namely 2RL22, does not. Here we have characterised the hematopoietic support capacity of each stroma, and analysed lineage origin of the stromal cell lines by gene profiling using microarrays. Stromal co-culture experiments were performed using bone marrow cells...[Show more]

dc.contributor.authorDespars, Genevieve
dc.contributor.authorPeriasamy, Pravin
dc.contributor.authorTan, Jonathan
dc.contributor.authorAbbey, Janice
dc.contributor.authorO'Neill, Terence
dc.contributor.authorO'Neill, Helen
dc.date.accessioned2015-12-08T22:45:57Z
dc.identifier.issn1547-3287
dc.identifier.urihttp://hdl.handle.net/1885/37932
dc.description.abstractSpleen stromal cells are critical determinants of dendritic cell (DC) development in spleen. The spleen stromal line, namely STX3, supports DC differentiation in vitro from overlaid bone marrow cells while the lymph node stromal line, namely 2RL22, does not. Here we have characterised the hematopoietic support capacity of each stroma, and analysed lineage origin of the stromal cell lines by gene profiling using microarrays. Stromal co-culture experiments were performed using bone marrow cells as a source of hematopoietic progenitors. A characteristic immature myeloid-like CD11c+CD11b +CD86+MHC-II-/loB220-CD8α - DC is produced after 14 days in STX3 cocultures, while 2RL22 cocultures produce only monocyte/macrophage-like cells. No other hematopoietic cell type is produced. The STX3 and 2RL22 stroma were compared by transcriptome analysis utilising Affymetrix Murine U74Av2 genechips to identify gene expression related to differential hematopoietic support function. Data mining was used to determine cell surface marker expression reflecting endothelial cells and fibroblasts, as well as adhesion molecules contributing to the microenvironment. STX3 shows gene expression reflective of early endothelial cells, while 2RL22 expresses markers specific to fibroblasts. The expression of genes like Flt1, CD34, Mcam, and Eng distinguishes STX3 as an early immature endothelial cell lacking markers of angioblasts or hemangioblasts like Tal1/SCL, Tie1, Tie2, Kdr or Prom1/AC133. The absence of expression of genes like Vwf and Cd31 distinguishes STX3 from fully differentiated vascular endothelial cells. In contrast, the 2RL22 lymph node stroma specifically expresses genes related to fibroblastic-like cells like osteoblasts with expression of Vdr (Vitamin D receptor), and epithelial cells with expression of Krt13 (keratins). Gene expression data identifies STX3 as splenic endothelial cells, independently able to support the outgrowth of immature, myeloid DC-like cells from progenitors present in bone marrow, while 2RL22 lymph node fibroblastic cells provide support for development of monocytes/macrophages.
dc.publisherMary Ann Liebert Inc.
dc.sourceStem Cells and Development
dc.subjectKeywords: angiopoietin receptor; CD11b antigen; CD133 antigen; CD146 antigen; CD31 antigen; CD34 antigen; CD45 antigen; CD86 antigen; CD8alpha antigen; cytokeratin 13; glycoprotein p 15095; major histocompatibility antigen class 2; Tie 1 receptor; transcription fac
dc.titleGene signature of Stromal Cells which support Dendritic Cell Development
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume17
dc.date.issued2008
local.identifier.absfor110799 - Immunology not elsewhere classified
local.identifier.ariespublicationu4325460xPUB155
local.type.statusPublished Version
local.contributor.affiliationDespars, Genevieve, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPeriasamy, Pravin, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationTan, Jonathan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationAbbey, Janice, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationO'Neill, Terence, College of Business and Economics, ANU
local.contributor.affiliationO'Neill, Helen, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage917
local.bibliographicCitation.lastpage928
local.identifier.doi10.1089/scd.2007.0170
dc.date.updated2016-02-24T10:54:25Z
local.identifier.scopusID2-s2.0-58149147393
local.identifier.thomsonID000260190100009
CollectionsANU Research Publications

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