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Putative Functional Role for the Invariant Aspartate 263 Residue of Rhodospirillum rubrum Rubisco

Liggins, John; Gready, Jill

Description

Although aspartate residue D263 of Rhodospirillum rubrum Rubisco is close to the active site and invariant in all reported Rubiscos, its possible functional and structural roles in Rubisco activity have not been investigated. We have mutagenised D263 to several selected amino acids (asparagine, alanine, serine, glutamate, and glutamine) to probe possible roles in facilitating proton movements within the active site and maintaining structural positioning of key active-site groups. The mutants...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
URI: http://hdl.handle.net/1885/37443
Source: Biochemistry
DOI: 10.1021/bi802159e

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