Identification of active site residues in the Shigella flexneri glucosyltransferase GtrV
The serotype-specific glucosyltransferase, GtrV, is responsible for glucosylation of the O-antigen repeating unit of Shigella flexneri serotype 5a strains. GtrV is an integral inner membrane protein with two essential periplasmic loops: the large Loop 2 and the C-terminal Loop 10. In this study, the full length of the Loop 2 was shown to be necessary for GtrV function. Site-directed mutagenesis within this loop revealed that conserved aromatic and charged amino acids have a critical role in the...[Show more]
|Collections||ANU Research Publications|
|Source:||Molecular Membrane Biology|
|01_Moscoso_Identification_of_active_site_2010.pdf||7.85 MB||Adobe PDF||Request a copy|
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