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Incidence of Myoendothelial Gap Junctions in the Proximal and Distal Mesenteric Arteries of the Rat Is Suggestive of a Role in Endothelium-Derived Hyperpolarizing Factor - Mediated Responses

Sandow, Shaun L; Hill, Caryl

Description

Although the chemical nature of endothelium-derived hyperpolarizing factor (EDHF) remains elusive, electrophysiological evidence exists for electrical communication between smooth muscle cells and endothelial cells suggesting that electrotonic propagation of hyperpolarization may explain the failure to identify a single chemical factor as EDHF. Anatomical evidence for myoendothelial gap junctions, or the sites of electrical coupling, is, however, rare. In the present study, serial-section...[Show more]

dc.contributor.authorSandow, Shaun L
dc.contributor.authorHill, Caryl
dc.date.accessioned2015-12-08T22:37:46Z
dc.identifier.issn0009-7330
dc.identifier.urihttp://hdl.handle.net/1885/35658
dc.description.abstractAlthough the chemical nature of endothelium-derived hyperpolarizing factor (EDHF) remains elusive, electrophysiological evidence exists for electrical communication between smooth muscle cells and endothelial cells suggesting that electrotonic propagation of hyperpolarization may explain the failure to identify a single chemical factor as EDHF. Anatomical evidence for myoendothelial gap junctions, or the sites of electrical coupling, is, however, rare. In the present study, serial-section electron microscopy and reconstruction techniques have been used to examine the incidence of myoendothelial gap junctions in the proximal and distal mesenteric arteries of the rat where EDHF responses have been reported to vary. Myoendothelial gap junctions were found to be very small in the mesenteric arteries, the majority being <100 nm in diameter. In addition, they were significantly more common in the distal compared with the proximal regions of this arterial bed. Pentalaminar gap junctions between adjacent endothelial cells were much larger and were common in both proximal and distal mesenteric arteries. These latter junctions were frequently found near the myoendothelial gap junctions. These results provide the first evidence for the presence of sites for electrical communication between endothelial cells and smooth muscle cells in the mesenteric vascular bed. Furthermore, the relative incidence of these sites suggests that there may be a relationship between the activity of EDHF and the presence of myoendothelial gap junctions.
dc.publisherLippincott Williams & Wilkins
dc.sourceCirculation Research
dc.subjectKeywords: endothelium derived hyperpolarizing factor; animal cell; article; binding site; cell interaction; cell transport; endothelium cell; gap junction; incidence; mesenteric artery; nonhuman; priority journal; rat; smooth muscle fiber; Animals; Biological Facto Endothelium; Smooth muscle; Three- dimensional reconstruction; Ultrastructure; Vascular Communication
dc.titleIncidence of Myoendothelial Gap Junctions in the Proximal and Distal Mesenteric Arteries of the Rat Is Suggestive of a Role in Endothelium-Derived Hyperpolarizing Factor - Mediated Responses
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume86
dc.date.issued2000
local.identifier.absfor110201 - Cardiology (incl. Cardiovascular Diseases)
local.identifier.ariespublicationMigratedxPub127
local.type.statusPublished Version
local.contributor.affiliationSandow, Shaun L, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHill, Caryl, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage341
local.bibliographicCitation.lastpage346
dc.date.updated2015-12-08T10:02:29Z
local.identifier.scopusID2-s2.0-0033956534
CollectionsANU Research Publications

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