Immunohistochemical expression of EGFR in colorectal carcinoma correlates with high but not low level gene amplification, as demonstrated by CISH
Aim: To assess and compare immunohistochemical expression of epidermal growth factor receptor EGFR with gene amplification as demonstrated by chromogenic in situ hybridisation CISH, in colorectal adenocarcinoma. Methods: Sections from 100 consecutive colorectal cancer resection specimens were stained for EGFR using immunohistochemistry and CISH. Immunohistochemical assessment was independently performed at two laboratories, using the same antibody and protocols. Results: With...[Show more]
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