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The Coenzyme A Biosynthetic Enzyme Phosphopantetheine Adenylyltransferase Plays a Crucial Role in Plant Growth, Salt/Osmotic Stress Resistance, and Seed Lipid Storage

Rubio, Silvia; Whitehead, Lynne; Larson, Tony; Graham, Ian A; Rodriguez, Pedro L

Description

Coenzyme A (CoA) is an essential cofactor in the metabolism of both prokaryotic and eukaryotic organisms and a universal five-step pathway is utilized to synthesize CoA from pantothenate. Null mutations in two of the five steps of this pathway led to embryo lethality and therefore viable reduction-of-function mutations are required to further study its role in plant biology. In this article, we have characterized a viable Arabidopsis (Arabidopsis thaliana) T-DNA mutant affected in the...[Show more]

dc.contributor.authorRubio, Silvia
dc.contributor.authorWhitehead, Lynne
dc.contributor.authorLarson, Tony
dc.contributor.authorGraham, Ian A
dc.contributor.authorRodriguez, Pedro L
dc.date.accessioned2015-12-08T22:35:48Z
dc.identifier.issn0032-0889
dc.identifier.urihttp://hdl.handle.net/1885/35008
dc.description.abstractCoenzyme A (CoA) is an essential cofactor in the metabolism of both prokaryotic and eukaryotic organisms and a universal five-step pathway is utilized to synthesize CoA from pantothenate. Null mutations in two of the five steps of this pathway led to embryo lethality and therefore viable reduction-of-function mutations are required to further study its role in plant biology. In this article, we have characterized a viable Arabidopsis (Arabidopsis thaliana) T-DNA mutant affected in the penultimate step of the CoA biosynthesis pathway, which is catalyzed by the enzyme phosphopantetheine adenylyltransferase (PPAT). This ppat-1 knockdown mutation showed an approximately 90% reduction in PPAT transcript levels and was severely impaired in plant growth and seed production. The sum of CoA and acetyl-CoA levels was severely reduced (60%-80%) in ppat-1 seedlings compared to wild type, and catabolism of storage lipids during seedling establishment was delayed. Conversely, PPAT overexpressing lines showed, on average, approximately 1.6-fold higher levels of CoA + acetyl-CoA levels, as well as enhanced vegetative and reproductive growth and salt/osmotic stress resistance. Interestingly, dry seeds of overexpressing lines contained between 35% to 50% more fatty acids than wild type, which suggests that CoA biosynthesis plays a crucial role in storage oil accumulation. Finally, biochemical analysis of the recombinant PPAT enzyme revealed an inhibitory effect of CoA on PPAT activity. Taken together, these results suggest that the reaction catalyzed by PPAT is a regulatory step in the CoA biosynthetic pathway that plays a key role for plant growth, stress resistance, and seed lipid storage.
dc.publisherAmerican Society of Plant Biologists
dc.sourcePlant Physiology
dc.subjectKeywords: Arabidopsis; Arabidopsis thaliana; Eukaryota; Prokaryota; acyl coenzyme A; fatty acid; nucleotidyltransferase; pantetheine phosphate adenylyltransferase; pantetheine-phosphate adenylyltransferase; Arabidopsis; article; gene expression regulation; genetics
dc.titleThe Coenzyme A Biosynthetic Enzyme Phosphopantetheine Adenylyltransferase Plays a Crucial Role in Plant Growth, Salt/Osmotic Stress Resistance, and Seed Lipid Storage
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume148
dc.date.issued2008
local.identifier.absfor060104 - Cell Metabolism
local.identifier.ariespublicationu4956746xPUB119
local.type.statusPublished Version
local.contributor.affiliationRubio, Silvia, Universidad Polite┬┤cnica de Valencia
local.contributor.affiliationWhitehead, Lynne, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLarson, Tony, University of York
local.contributor.affiliationGraham, Ian A, Univeristy of York
local.contributor.affiliationRodriguez, Pedro L, Universidad Polite┬┤cnica de Valencia
local.description.embargo2037-12-31
local.bibliographicCitation.startpage546
local.bibliographicCitation.lastpage556
local.identifier.doi10.1104/pp.108.124057
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T11:26:50Z
local.identifier.scopusID2-s2.0-55549098574
local.identifier.thomsonID000258947600047
CollectionsANU Research Publications

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