Efficient gene delivery using reconstituted chromatin enhanced for nuclear targeting
Nonviral gene delivery is hampered by difficulties associated with transporting negatively charged DNA through the cell membrane and, more importantly, the nuclear envelope of target cells. Here we show for the first time that chromatin reconstituted with histone H2B proteins optimized for nuclear targeting can be used as an efficient means to deliver DNA to the nucleus of intact living mammalian cells, resulting in high levels of transgene expression that were ∼6-fold more than those achieved...[Show more]
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|Source:||The FASEB journal : official publication of the Federation of American Societies for Experimental Biology|
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