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Simultaneous multi-site two-photon photostimulation in three dimensions

Go, Mary-Ann; Stricker, Christian; Redman, Stephen; Bachor, Hans; Daria, Vincent

Description

We demonstrate simultaneous multi-site two-photon photolysis of caged neurotransmitters with close to diffraction-limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two-photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its...[Show more]

dc.contributor.authorGo, Mary-Ann
dc.contributor.authorStricker, Christian
dc.contributor.authorRedman, Stephen
dc.contributor.authorBachor, Hans
dc.contributor.authorDaria, Vincent
dc.date.accessioned2015-12-08T22:24:51Z
dc.identifier.issn1864-063X
dc.identifier.urihttp://hdl.handle.net/1885/33171
dc.description.abstractWe demonstrate simultaneous multi-site two-photon photolysis of caged neurotransmitters with close to diffraction-limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two-photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its application to studies of synaptic integration by performing simultaneous and controlled glutamate delivery at multiple locations on dendritic trees.
dc.publisherWiley-VCH Verlag GMBH
dc.sourceJournal of Biophotonics
dc.subjectKeywords: 3D morphology; 3D positions; Diffraction-limited resolution; Focal spot; Full control; Holographic projection; Localized excitations; Multi-site; Photostimulation; Synaptic integration; Three dimensions; Two photon; Two photon imaging; Photolysis; Three d Holographic projection; Synaptic integration; Two-photon imaging; Two-photon photolysis
dc.titleSimultaneous multi-site two-photon photostimulation in three dimensions
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolumeOnline
dc.date.issued2012
local.identifier.absfor069999 - Biological Sciences not elsewhere classified
local.identifier.ariespublicationu4693331xPUB99
local.type.statusPublished Version
local.contributor.affiliationGo, Mary-Ann, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationStricker, Christian, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRedman, Stephen, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBachor, Hans, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationDaria, Vincent, College of Physical and Mathematical Sciences, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage9
local.identifier.doi10.1002/jbio.201100101
dc.date.updated2016-02-24T11:18:19Z
local.identifier.scopusID2-s2.0-84861953358
local.identifier.thomsonID000309452400009
CollectionsANU Research Publications

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