Go, Mary-Ann; Stricker, Christian; Redman, Stephen; Bachor, Hans; Daria, Vincent
We demonstrate simultaneous multi-site two-photon photolysis of caged neurotransmitters with close to diffraction-limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two-photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its...[Show more]
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.