Understanding the physics of oligonucleotide microarrays: the Affymetrix spike-in data reanalysed
The Affymetrix U95 and U133 Latin-Square spike-in datasets are reanalysed, together with a dataset from a version of the U95 spike-in experiment without a complex non-specific background. The approach uses a physico-chemical model which includes the effects of the specific and non-specific hybridization and probe folding at the microarray surface, target folding and hybridization in the bulk RNA target solution and duplex dissociation during the post-hybridization washing phase. The model...[Show more]
|Collections||ANU Research Publications|
|01_Burden_Understanding_the_physics_of_2008.pdf||1.25 MB||Adobe PDF||Request a copy|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.