A Potential Food-Grade Cloning Vector for Streptococcus thermophilus That Uses Cadmium Resistance as the Selectable Marker
A potential food-grade cloning vector, pND919, was constructed and transformed into S. thermophilus ST3-1, a plasmid-free strain. The vector contains DNAs from two different food-approved organisms, Streptococcus thermophilus and Lactococcus lactis. The 5.0-kb pND919 is a derivative of the cloning vector pND918 (9.3 kb) and was constructed by deletion of the 4.3-kb region of pND918 which contained DNA from non- food-approved organisms. pND919 carries a heterologous native cadmium resistance...[Show more]
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|Source:||Applied and Environmental Microbiology|
|01_Wong_A_Potential_Food-Grade_Cloning_2003.pdf||214.49 kB||Adobe PDF||Request a copy|
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