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A Potential Food-Grade Cloning Vector for Streptococcus thermophilus That Uses Cadmium Resistance as the Selectable Marker

Wong, Wing Yee; Su, Ping; Allison, Gwen; Liu, Chun-Qiang; Dunn, Noel W


A potential food-grade cloning vector, pND919, was constructed and transformed into S. thermophilus ST3-1, a plasmid-free strain. The vector contains DNAs from two different food-approved organisms, Streptococcus thermophilus and Lactococcus lactis. The 5.0-kb pND919 is a derivative of the cloning vector pND918 (9.3 kb) and was constructed by deletion of the 4.3-kb region of pND918 which contained DNA from non- food-approved organisms. pND919 carries a heterologous native cadmium resistance...[Show more]

CollectionsANU Research Publications
Date published: 2003
Type: Journal article
Source: Applied and Environmental Microbiology
DOI: 10.1128/AEM.69.10.5767-5771.2003


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