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Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit

Chung, Wen Yuan; Sack, Markus; Carter, Rachel; Spiegel, Holger; Fischer, Rainer; Hirst, Timothy; Williams, Neil; James, Roger


Previously we have described studies on in vitro pentamer assembly of Escherichia coli (E. coli) derived heat-labile enterotoxin B subunit (EtxB) using conventional monoclonal antibodies (Amin et al., JBC 1995: 270, 20143-50 and Chung et al., JBC 2006: 281, 39465-70). To extend these studies further we have used phage-display to select single-chain Fragment variable (scFv) antibodies against different forms of the B-subunit. Two clones exhibiting strong and differential binding were chosen for...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
Source: Journal of Immunological Methods
DOI: 10.1016/j.jim.2008.08.005


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