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Effects of bacterial products on enterocyte-macrophage interactions in vitro

Tyrer, Peter; Bean, Elaine; Foxwell, A.R; Pavli, Paul


We describe a coculture model of a human intestinal epithelial cell line and human peripheral blood monocytes in which monocytes differentiate into cells with features of resident intestinal macrophages. Caco-2 cells are grown on the lower surface of a semipermeable filter with pore size of 3μm (Transwells®) until they differentiate into enterocytes. Peripheral-blood monocytes are added and the co-culture incubated for two days. Monocytes migrate through the pores of the membrane, come into...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
Source: Biochemical and Biophysical Research Communications
DOI: 10.1016/j.bbrc.2011.08.100


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