Effects of bacterial products on enterocyte-macrophage interactions in vitro
We describe a coculture model of a human intestinal epithelial cell line and human peripheral blood monocytes in which monocytes differentiate into cells with features of resident intestinal macrophages. Caco-2 cells are grown on the lower surface of a semipermeable filter with pore size of 3μm (Transwells®) until they differentiate into enterocytes. Peripheral-blood monocytes are added and the co-culture incubated for two days. Monocytes migrate through the pores of the membrane, come into...[Show more]
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|Source:||Biochemical and Biophysical Research Communications|
|01_Tyrer_Effects_of_bacterial_products_2011.pdf||360.24 kB||Adobe PDF||Request a copy|
|02_Tyrer_Effects_of_bacterial_products_2011.pdf||1.2 MB||Adobe PDF||Request a copy|
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