Significance of the 2-O-sulfo group of L-iduronic acid residues in heparin on the growth inhibition of bovine pulmonary artery smooth muscle cells
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Garg, Hari; Mrabat, Hicham; Yu, Lunyin; Freeman, Craig; Boyangzi, Li; Zhang, Fuming; Linhardt, Robert J; Hales, Charles A.
Description
Heparin inhibits the growth of several cell types in vitro, including bovine pulmonary artery smooth muscle cells (BPASMCs). To understand more about the heparin structure required for endogenous activity, chemically modified derivatives of native heparin and glycol-split heparin, namely, 2-O-desulfonated iduronic/glucuronic acid residues in heparin, and 2-O-desulfonated iduronic residues in glycol-split heparin were prepared. These were assayed for their antiproliferative potency on cultured...[Show more]
dc.contributor.author | Garg, Hari | |
---|---|---|
dc.contributor.author | Mrabat, Hicham | |
dc.contributor.author | Yu, Lunyin | |
dc.contributor.author | Freeman, Craig | |
dc.contributor.author | Boyangzi, Li | |
dc.contributor.author | Zhang, Fuming | |
dc.contributor.author | Linhardt, Robert J | |
dc.contributor.author | Hales, Charles A. | |
dc.date.accessioned | 2015-12-08T22:20:23Z | |
dc.identifier.issn | 0008-6215 | |
dc.identifier.uri | http://hdl.handle.net/1885/31970 | |
dc.description.abstract | Heparin inhibits the growth of several cell types in vitro, including bovine pulmonary artery smooth muscle cells (BPASMCs). To understand more about the heparin structure required for endogenous activity, chemically modified derivatives of native heparin and glycol-split heparin, namely, 2-O-desulfonated iduronic/glucuronic acid residues in heparin, and 2-O-desulfonated iduronic residues in glycol-split heparin were prepared. These were assayed for their antiproliferative potency on cultured BPASMCs. All of the 2-O-desulfonated heparin derivatives had significantly decreased less antiproliferative activity on BPASMCs. These results suggest that the 2-O-sulfo group of iduronic acid residues in heparin's major sequence is essential for the antiproliferative properties of heparin. The size of heparin does not affect the growth-inhibitory properties of heparin on BPASMCs at the three dose levels examined. | |
dc.publisher | Elsevier | |
dc.source | Carbohydrate Research | |
dc.subject | Keywords: Cell growth; Cells; Ethylene glycol; Glycols; Muscle; Acid residues; Anti-proliferative; Antiproliferative activities; Antiproliferative properties; Cell proliferation; Cell types; Desulfated heparin; Dose levels; Growth inhibition; Heparin; Iduronic acid Cell proliferation; Desulfated heparin; Heparin | |
dc.title | Significance of the 2-O-sulfo group of L-iduronic acid residues in heparin on the growth inhibition of bovine pulmonary artery smooth muscle cells | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 343 | |
dc.date.issued | 2008 | |
local.identifier.absfor | 110102 - Medical Biochemistry: Carbohydrates | |
local.identifier.ariespublication | u6800332xPUB87 | |
local.type.status | Published Version | |
local.contributor.affiliation | Garg, Hari, Harvard Medical School | |
local.contributor.affiliation | Mrabat, Hicham, Harvard Medical School | |
local.contributor.affiliation | Yu, Lunyin, Harvard Medical School | |
local.contributor.affiliation | Freeman, Craig, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Boyangzi, Li, Rensselaer Polytechnic Institute | |
local.contributor.affiliation | Zhang, Fuming, Rensselaer Polytechnic Institute | |
local.contributor.affiliation | Linhardt, Robert J, Rensselaer Polytechnic Institute | |
local.contributor.affiliation | Hales, Charles A., Harvard Medical School | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 2406 | |
local.bibliographicCitation.lastpage | 2410 | |
local.identifier.doi | 10.1016/j.carres.2008.06.022 | |
dc.date.updated | 2015-12-08T08:30:11Z | |
local.identifier.scopusID | 2-s2.0-49649093283 | |
local.identifier.thomsonID | 000259797200008 | |
Collections | ANU Research Publications |
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