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Increased hypothalamic GPR54 signaling: a potential mechanism for initiation of puberty in primates

Shahab, Muhammad; Mastronardi, Claudio; Seminara, Stephanie B.; Crowley, William F.; Ojeda, Sergio R; Plant, Tony M.

Description

To further study the role of GPR54 signaling in the onset of primate puberty, we used the monkey to examine the ability of kisspeptin-10 to elicit the release of gonadotropin-releasing hormone (GnRH) precociously, and we describe the expression of GPR54 and KiSS-1 in the hypothalamus during the peripubertal period. Agonadal juvenile male monkeys were implanted with a lateral cerebroventricular cannula and a jugular vein catheter. The responsiveness of the juvenile pituitary to endogenous GnRH...[Show more]

dc.contributor.authorShahab, Muhammad
dc.contributor.authorMastronardi, Claudio
dc.contributor.authorSeminara, Stephanie B.
dc.contributor.authorCrowley, William F.
dc.contributor.authorOjeda, Sergio R
dc.contributor.authorPlant, Tony M.
dc.date.accessioned2015-12-08T22:18:49Z
dc.identifier.issn0027-8424
dc.identifier.urihttp://hdl.handle.net/1885/31523
dc.description.abstractTo further study the role of GPR54 signaling in the onset of primate puberty, we used the monkey to examine the ability of kisspeptin-10 to elicit the release of gonadotropin-releasing hormone (GnRH) precociously, and we describe the expression of GPR54 and KiSS-1 in the hypothalamus during the peripubertal period. Agonadal juvenile male monkeys were implanted with a lateral cerebroventricular cannula and a jugular vein catheter. The responsiveness of the juvenile pituitary to endogenous GnRH release was heightened with a chronic pulsatile i.v. infusion of synthetic GnRH before kisspeptin-10 (112-121) injection. Intracerebroventricular (30 μg or 100 μg) or i.v. (100 μg) bolus injections of kisspeptin-10 elicited a robust GnRM discharge, as reflected by luteinizing hormone secretion, which was abolished by pretreatment with a GnRH-receptor antagonist. RNA was isolated from the hypothalamus of agonadal males before (juvenile) and after (pubertal) the pubertal resurgence of pulsatile GnRH release and from juvenile, early pubertal, and midpubertal ovary-intact females. KiSS-1 mRNA levels detected by real-time PCR increased with puberty in both male and female monkeys. In intact females, but not in agonadal males, GPR54 mRNA levels in the hypothalamus increased ≈ 3-fold from the juvenile to midpubertal stage. Hybridization histochemistry indicated robust KiSS-1 and GPR54 mRNA expression in the region of the arcuate nucleus. These findings are consistent with the hypothesis that GPR54 signaling by its cognate ligand in the primate hypothalamus may be activated at the end of the juvenile phase of development and may contribute to the pubertal resurgence of pulsatile GnRH release, the central drive for puberty.
dc.publisherNational Academy of Sciences (USA)
dc.sourcePNAS - Proceedings of the National Academy of Sciences of the United States of America
dc.subjectKeywords: blocking agent; G protein coupled receptor; G protein coupled receptor 54; gonadorelin receptor; gonadorelin receptor antagonist; kisspeptin 10; messenger RNA; RNA; unclassified drug; animal experiment; animal tissue; arcuate nucleus; article; cannula; co Development; Gonadotropin-releasing hormone; GPR54; Hypothalamus; Kisspeptins; Monkey
dc.titleIncreased hypothalamic GPR54 signaling: a potential mechanism for initiation of puberty in primates
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume102
dc.date.issued2005
local.identifier.absfor110999 - Neurosciences not elsewhere classified
local.identifier.ariespublicationu9505948xPUB83
local.type.statusPublished Version
local.contributor.affiliationShahab, Muhammad , University of Pittsburgh School of Medicine
local.contributor.affiliationMastronardi, Claudio, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSeminara, Stephanie B., Massachusetts General Hospital
local.contributor.affiliationCrowley, William F., Massachusetts General Hospital,
local.contributor.affiliationOjeda, Sergio R, Oregan Health and Science University
local.contributor.affiliationPlant, Tony M., University of Pittsburgh School of Medicine
local.description.embargo2037-12-31
local.bibliographicCitation.issue6
local.bibliographicCitation.startpage2129
local.bibliographicCitation.lastpage2134
local.identifier.doi10.1073/pnas.0409822102
dc.date.updated2015-12-08T08:19:33Z
local.identifier.scopusID2-s2.0-13844317911
CollectionsANU Research Publications

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