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Translational incorporation of modified phenylalanines and tyrosines during cell-free protein synthesis

Wang, Zhongqiang; Matthews, Hayden


Inherent promiscuity of bacterial translation is demonstrated by mass spectrometric quantification of the translational incorporation of ring-substituted phenylalanines and tyrosines bearing fluoro-, hydroxyl-, methyl-, chloro- and nitro-groups in an E. coli-derived cell-free system. Competitive studies using the cell-free system show that the aminoacyl-tRNA synthetases (aaRS) have at least two orders of magnitude higher specificity for the native substrate over these structural analogues,...[Show more]

CollectionsANU Research Publications
Date published: 2020
Type: Journal article
Source: RSC Advances
DOI: 10.1039/d0ra00655f
Access Rights: Open Access


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