Rapid downregulation of the rat glutamine transporter SNAT3 by a caveolin-dependent trafficking mechanism in Xenopus laevis oocytes
-
Altmetric Citations
Balkrishna, Sarojini; Broer, Angelika; Kingsland, Alice; Broer, Stefan
Description
The glutamine transporter SNAT3 is involved in the uptake and release of glutamine in the brain, liver, and kidney. Substrate transport is accompanied by Na+ cotransport and H+ antiport. In this study, treatment of Xenopus laevis oocytes expressing rat SNAT3 with the phorbol ester PMA resulted in a rapid downregulation of glutamine uptake in less than 20 min. PMA treatment of oocytes coexpressing SNAT3 and the monocarboxylate transporter MCT1 reduced SNAT3 activity only, demonstrating the...[Show more]
dc.contributor.author | Balkrishna, Sarojini | |
---|---|---|
dc.contributor.author | Broer, Angelika | |
dc.contributor.author | Kingsland, Alice | |
dc.contributor.author | Broer, Stefan | |
dc.date.accessioned | 2015-12-08T22:18:18Z | |
dc.identifier.issn | 0363-6143 | |
dc.identifier.uri | http://hdl.handle.net/1885/31286 | |
dc.description.abstract | The glutamine transporter SNAT3 is involved in the uptake and release of glutamine in the brain, liver, and kidney. Substrate transport is accompanied by Na+ cotransport and H+ antiport. In this study, treatment of Xenopus laevis oocytes expressing rat SNAT3 with the phorbol ester PMA resulted in a rapid downregulation of glutamine uptake in less than 20 min. PMA treatment of oocytes coexpressing SNAT3 and the monocarboxylate transporter MCT1 reduced SNAT3 activity only, demonstrating the specificity of the regulatory mechanism. Single or combined mutations of seven putative phosphorylation sites in the SNAT3 sequence did not affect the regulation of SNAT3 by PMA. Expression of an EGFP-SNAT3 fusion protein in oocytes established that the down-regulation was caused by the retrieval of the transporter from the plasma membrane. Coexpression of SNAT3 with dominant-negative mutants of dynamin or caveolin revealed that SNAT3 trafficking occurs in a dynamin-independent manner and is influenced by caveolin. Although system N activity was not affected by PMA in cultured astrocytes, a downregulation was observed in HepG2 cells. | |
dc.publisher | American Physiological Society | |
dc.source | American Journal of Physiology - Cell Physiology | |
dc.subject | Keywords: carrier protein; caveolin; dynamin; monocarboxylate transporter 1; mutant protein; protein snat3; unclassified drug; animal cell; article; controlled study; down regulation; female; newborn; nonhuman; oocyte; priority journal; protein phosphorylation; pro Glutamine transport; Phorbol ester; Protein kinase C; Protein trafficking; SLC38A3 | |
dc.title | Rapid downregulation of the rat glutamine transporter SNAT3 by a caveolin-dependent trafficking mechanism in Xenopus laevis oocytes | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 299 | |
dc.date.issued | 2010 | |
local.identifier.absfor | 060110 - Receptors and Membrane Biology | |
local.identifier.ariespublication | u8611701xPUB81 | |
local.type.status | Published Version | |
local.contributor.affiliation | Balkrishna, Sarojini, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Broer, Angelika, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Kingsland, Alice, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Broer, Stefan, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | C1047 | |
local.bibliographicCitation.lastpage | C1057 | |
local.identifier.doi | 10.1152/ajpcell.00209.2010 | |
local.identifier.absseo | 920105 - Digestive System Disorders | |
dc.date.updated | 2016-02-24T11:42:32Z | |
local.identifier.scopusID | 2-s2.0-78349290358 | |
local.identifier.thomsonID | 000283604400022 | |
Collections | ANU Research Publications |
Download
File | Description | Size | Format | Image |
---|---|---|---|---|
01_Balkrishna_Rapid_downregulation_of_the_2010.pdf | 983.66 kB | Adobe PDF | Request a copy |
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.
Updated: 17 November 2022/ Responsible Officer: University Librarian/ Page Contact: Library Systems & Web Coordinator