The innate immune response to adjuvants dictates the adaptive immune response to autoantigens
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Staykova, Maria; Linares, David; Fordham, Susan; Paridaen, Judith T; Willenborg, David
Description
To elucidate the role of innate immunity in susceptibility to the animal model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE), we induced EAE by immunization with spinal cord homogenate (SCH) plus complete Freund adjuvant or carbonyl iron in 3 inbred rat strains. Lewis are considered "susceptible," PVG/c-Rt7a (PVG) as "semisusceptible," and Brown Norway (BN) as "resistant" to EAE. Immunization with SCH-carbonyl iron resulted in clinical disease in all 3 strains, but the...[Show more]
dc.contributor.author | Staykova, Maria | |
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dc.contributor.author | Linares, David | |
dc.contributor.author | Fordham, Susan | |
dc.contributor.author | Paridaen, Judith T | |
dc.contributor.author | Willenborg, David | |
dc.date.accessioned | 2015-12-08T22:18:04Z | |
dc.identifier.issn | 0022-3069 | |
dc.identifier.uri | http://hdl.handle.net/1885/31180 | |
dc.description.abstract | To elucidate the role of innate immunity in susceptibility to the animal model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE), we induced EAE by immunization with spinal cord homogenate (SCH) plus complete Freund adjuvant or carbonyl iron in 3 inbred rat strains. Lewis are considered "susceptible," PVG/c-Rt7a (PVG) as "semisusceptible," and Brown Norway (BN) as "resistant" to EAE. Immunization with SCH-carbonyl iron resulted in clinical disease in all 3 strains, but the pathologic features of EAE in the resistant BN and the semisusceptible PVG rats differed from those in the Lewis and PVG model of EAE induced with SCH-complete Freund adjuvant. In BN and PVG rats, there were numerous inflammatory lesions with prominent involvement of microglia and, to a lesser extent, perivascular macrophages. These data suggest that different levels of activation of the innate immune system by different adjuvants determine whether EAE will or will not develop. Accordingly, the widely accepted scale of susceptibility to EAE development (Lewis > PVG > BN) should be revised because it does not take into account the important contribution of the composition of the adjuvant to the quality and quantity of the innate immune response and, consequently, to the generation and extent of the pathogenic T-cell-mediated, that is, adaptive, autoimmune disease. | |
dc.publisher | Lippincott Williams & Wilkins | |
dc.source | Journal of Neuropathology and Experimental Neurology | |
dc.subject | Keywords: autoantigen; Freund adjuvant; adaptive immunity; allergic encephalomyelitis; animal experiment; animal model; article; controlled study; female; homogenate; immunization; inflammation; innate immunity; microglia; multiple sclerosis; nonhuman; priority jou Autoimmune; Experimental autoimmune encephalomyelitis; Innate immunity; Microglia; Multiple sclerosis; Nitric oxide | |
dc.title | The innate immune response to adjuvants dictates the adaptive immune response to autoantigens | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 67 | |
dc.date.issued | 2008 | |
local.identifier.absfor | 110904 - Neurology and Neuromuscular Diseases | |
local.identifier.ariespublication | u6800332xPUB80 | |
local.type.status | Published Version | |
local.contributor.affiliation | Staykova, Maria, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Linares, David, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Fordham, Sue, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Paridaen, Judith T, Utrecht University | |
local.contributor.affiliation | Willenborg, David, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 6 | |
local.bibliographicCitation.startpage | 543 | |
local.bibliographicCitation.lastpage | 554 | |
local.identifier.doi | 10.1097/NEN.0b013e31817713cc | |
dc.date.updated | 2015-12-08T08:12:54Z | |
local.identifier.scopusID | 2-s2.0-44649160704 | |
Collections | ANU Research Publications |
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