Determining RuBisCO activation kinetics and other rate and equilibrium constants by simultaneous multiple non-linear regression of a kinetic model
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McNevin, Dennis; von Caemmerer, Susanne; Farquhar, Graham
Description
The forward and reverse rate constants involved in carbamylation, activation, carboxylation, and inhibition of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) have been estimated by a new technique of simultaneous non-linear regression of a differential equation kinetic model to multiple experimental data. Parameters predicted by the model fitted to data from purified spinach enzyme in vitro included binding affinity constants for non-substrate CO2 and Mg2+ of 200±80 μM and 700±200...[Show more]
Collections | ANU Research Publications |
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Date published: | 2006 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/30969 |
Source: | Journal of Experimental Biology |
DOI: | 10.1093/jxb/erl156 |
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01_McNevin_Determining_RuBisCO_activation_2006.pdf | 589.78 kB | Adobe PDF |
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