Skip navigation
Skip navigation

Strain-dependent resistance to allergen-induced lung pathophysiology in mice correlates with rate of apoptosis of lung-derived eosinophils

Tumes, Damon J; Cormie, James; Calvert, Michael G; Stewart, Kalev; Nassenstein, Christina; Braun, Armin; Foster, Paul S; Dent, Lindsay

Description

Although exposed to similar allergic and environmental stimuli, not all humans develop asthma. Similarly, mouse strains vary in the degree of pathophysiology seen following induction of experimental asthma. Three mouse strains (CBA/Ca, BALB/c, and C57BL/6) were used to determine if the extent and duration of inflammation influenced the degree of lung tissue damage in an OVA-induced allergic asthma model. Airways obstruction, leukocyte infiltration, edema, eosinophil accumulation, and...[Show more]

dc.contributor.authorTumes, Damon J
dc.contributor.authorCormie, James
dc.contributor.authorCalvert, Michael G
dc.contributor.authorStewart, Kalev
dc.contributor.authorNassenstein, Christina
dc.contributor.authorBraun, Armin
dc.contributor.authorFoster, Paul S
dc.contributor.authorDent, Lindsay
dc.date.accessioned2015-12-08T22:16:41Z
dc.identifier.issn0741-5400
dc.identifier.urihttp://hdl.handle.net/1885/30783
dc.description.abstractAlthough exposed to similar allergic and environmental stimuli, not all humans develop asthma. Similarly, mouse strains vary in the degree of pathophysiology seen following induction of experimental asthma. Three mouse strains (CBA/Ca, BALB/c, and C57BL/6) were used to determine if the extent and duration of inflammation influenced the degree of lung tissue damage in an OVA-induced allergic asthma model. Airways obstruction, leukocyte infiltration, edema, eosinophil accumulation, and degranulation were less severe in wild-type (wt) CBA/Ca mice than wt BALB/c and C57BL/6 mice. F1 hybrids of CBA/Ca mice crossed with BALB/c or C57BL/6 mice had bronchoalveolar lavage leukocyte (BAL) and cell-free protein profiles similar to those of the respective disease-susceptible parental strain. IL-5 transgene expression on each of the three genetic backgrounds accentuated the difference between CBA/Ca and the other two strains. Importantly, even when overexpressing IL-5, CBA/Ca mice did not develop substantial airways obstruction. Eosinophils recovered from the airways of allergic wt and IL-5 transgenic (Tg) CBA/Ca mice entered apoptosis at a faster rate than eosinophils from the other parental strains and F1 hybrids. In contrast, eosinophils harvested from the peritoneal cavities of untreated CBA/Ca IL-5 Tg mice had a relatively low rate of apoptosis in vitro. The CBA/Ca mouse strain is therefore relatively resistant to experimental asthma, and this may be a consequence of a propensity for apoptosis of eosinophils recruited into the allergic lung. Restricting survival of a key effector cell may thus limit pathogenesis in this experimental model and in humans.
dc.publisherFederation of American Societies for Experimental Biology
dc.sourceJournal of Leukocyte Biology
dc.subjectKeywords: allergen; interleukin 5; airway obstruction; allergic asthma; analytic method; animal cell; animal experiment; animal model; apoptosis; article; cell infiltration; comparative study; controlled study; degranulation; disease predisposition; edema; eosinoph Allergy; Asthma; Inflammation; Interleukin 5; Necrosis
dc.titleStrain-dependent resistance to allergen-induced lung pathophysiology in mice correlates with rate of apoptosis of lung-derived eosinophils
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume81
dc.date.issued2007
local.identifier.absfor110203 - Respiratory Diseases
local.identifier.ariespublicationu4020362xPUB77
local.type.statusPublished Version
local.contributor.affiliationTumes, Damon J, University of Adelaide
local.contributor.affiliationCormie, James, University of Adelaide
local.contributor.affiliationCalvert, Michael G, University of Adelaide
local.contributor.affiliationStewart, Kalev, University of Adelaide
local.contributor.affiliationNassenstein, Christina, Fraunhofer Institute
local.contributor.affiliationBraun, Armin, Fraunhofer Institute
local.contributor.affiliationFoster, Paul S, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationDent, Lindsay, University of Adelaide
local.description.embargo2037-12-31
local.bibliographicCitation.issue6
local.bibliographicCitation.startpage1362
local.bibliographicCitation.lastpage73
local.identifier.doi10.1189/jlb.0106046
dc.date.updated2015-12-08T08:03:17Z
local.identifier.scopusID2-s2.0-34249653510
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Tumes_Strain-dependent_resistance_to_2007.pdf835.51 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator