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Quantitative assessment of intrinsic carbonic anhydrase activity and the capacity for bicarbonate oxidation in photosystem II

Hillier, Warwick; McConnell, Iain; Badger, Murray; Boussac, Alain; Klimov, Vyacheslav; Dismukes, G. Charles; Wydrzynski, Thomas

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On the basis of equilibrium isotopic distribution experiments using 18O-labeled water, it is generally accepted that water is the sole substrate for O2 production by photosystem II (PSII). Nevertheless, recent studies indicating a direct interaction between bicarbonate and the donor side of PSII have been used to hypothesize that bicarbonate may have been a physiologically important substrate for O2 production during the evolution of PSII [Dismukes, G. C., Klimov, V. V., Baranov, S. V., Kozlov,...[Show more]

dc.contributor.authorHillier, Warwick
dc.contributor.authorMcConnell, Iain
dc.contributor.authorBadger, Murray
dc.contributor.authorBoussac, Alain
dc.contributor.authorKlimov, Vyacheslav
dc.contributor.authorDismukes, G. Charles
dc.contributor.authorWydrzynski, Thomas
dc.date.accessioned2015-12-08T22:16:36Z
dc.identifier.issn0006-2960
dc.identifier.urihttp://hdl.handle.net/1885/30752
dc.description.abstractOn the basis of equilibrium isotopic distribution experiments using 18O-labeled water, it is generally accepted that water is the sole substrate for O2 production by photosystem II (PSII). Nevertheless, recent studies indicating a direct interaction between bicarbonate and the donor side of PSII have been used to hypothesize that bicarbonate may have been a physiologically important substrate for O2 production during the evolution of PSII [Dismukes, G. C., Klimov, V. V., Baranov, S. V., Kozlov, Y. N., DasGupta, J., and Tyryshikin, A. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 2170-2175]. To test out this hypothesis and to determine whether contemporary oxygenic organisms have the capacity to oxidize bicarbonate, we employed special rapid-mixing isotopic experiments using 18O/13C-labeled bicarbonate to quantify the inherent carbonic anhydrase activity in PSII samples and the potential flux of oxygen from bicarbonate into the photosynthetically produced O2. The measurements were made on PSII samples prepared from spinach, Thermosynechococcus elongatus, and Arthrospira maxima. For the latter organism, a strain was used that grows naturally in an alkaline, high (bi)carbonate soda lake in Africa. The results reveal that bicarbonate is not the substrate for O2 production in these contemporary oxygenic photoautotrophs when assayed under single turnover conditions.
dc.publisherAmerican Chemical Society
dc.sourceBiochemistry
dc.subjectKeywords: Bicycles; Carbon; Oxidation; Substrates; Water; Equilibrium isotopic distribution; Intrinsic carbonic anhydrase activity; O production; Photosystem II (PSII); Enzymes; bicarbonate; carbon 13; carbonate dehydratase; oxygen; oxygen 18; sodium carbonate; wat
dc.titleQuantitative assessment of intrinsic carbonic anhydrase activity and the capacity for bicarbonate oxidation in photosystem II
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume45
dc.date.issued2006
local.identifier.absfor060107 - Enzymes
local.identifier.ariespublicationu9204316xPUB76
local.type.statusPublished Version
local.contributor.affiliationHillier, Warwick, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMcConnell, Iain, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBadger, Murray, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBoussac, Alain , CEA-Saclay
local.contributor.affiliationKlimov, Vyacheslav, Russian Academy of Sciences
local.contributor.affiliationDismukes, G Charles, Princeton University
local.contributor.affiliationWydrzynski, Thomas, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage2094
local.bibliographicCitation.lastpage2102
local.identifier.doi10.1021/bi051892o
dc.date.updated2015-12-08T08:02:50Z
local.identifier.scopusID2-s2.0-33144472321
CollectionsANU Research Publications

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