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Patterned illumination for analyzing neuronal function in 3D

Go, Mary-Ann; Stricker, Christian; Redman, Stephen; Bachor, Hans; Daria, Vincent


We use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Conference paper
Source: Proceedings of Biophotonics: Photonic Solutions for Better Health Care Conference
DOI: 10.1117/12.923421


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