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A Chiral Lanthanide Tag for Stable and Rigid Attachment to Single Cysteine Residues in Proteins for NMR, EPR and Time-Resolved Luminescence Studies

Herath, Iresha D; Breen, Colum; Hewitt, Sarah H; Berki, Thomas R; Kassir, Ahmad F; Dodson, Charlotte; Judd, Martyna; Jabar, Shereen; Cox, Nicholas; Otting, Gottfried; Butler, Stephen J


A lanthanide-binding tag site-specifically attached to a protein presents a tool to probe the protein by multiple spectroscopic techniques, including nuclear magnetic resonance, electron paramagnetic resonance and time-resolved luminescence spectroscopy. Here a new stable chiral Ln(III) tag, referred to as C12, is presented for spontaneous and quantitative reaction with a cysteine residue to generate a stable thioether bond. The synthetic protocol of the tag is relatively straightforward, and...[Show more]

CollectionsANU Research Publications
Date published: 2021
Type: Journal article
Source: Chemistry, A European Journal
DOI: 10.1002/chem.202101143
Access Rights: Open Access


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