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A convenient method for preparation of an engineered mouse interleukin-3 analog with high solubility and wild-type bioactivity

Murphy, James M; Metcalf, Donald; Young, Ian; Hilton, Douglas J


Mouse interleukin-3 (mIL-3) is a critical cytokine regulator of myeloid cell differentiation, survival and activation, and consequently this cytokine has become a key reagent for hematological studies in the laboratory. Although bacterial expression has been used for the preparation of recombinant mIL-3 for more than 20 years, the resultant cytokine is known to exhibit poor solubility, be prone to aggregation, and may contain mispaired disulfide bonds. As a result, little structural...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
Source: Growth Factors
DOI: 10.3109/08977190903443048


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