A convenient method for preparation of an engineered mouse interleukin-3 analog with high solubility and wild-type bioactivity
Mouse interleukin-3 (mIL-3) is a critical cytokine regulator of myeloid cell differentiation, survival and activation, and consequently this cytokine has become a key reagent for hematological studies in the laboratory. Although bacterial expression has been used for the preparation of recombinant mIL-3 for more than 20 years, the resultant cytokine is known to exhibit poor solubility, be prone to aggregation, and may contain mispaired disulfide bonds. As a result, little structural...[Show more]
|Collections||ANU Research Publications|
|01_Murphy_A_convenient_method_for_2009.pdf||644.57 kB||Adobe PDF||Request a copy|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.